Why are you starting a new thread. It's very confusing. Now we have to go back and see what was said in the other thread to figure out where to start. Also, what does playing to sequence fine but effects are off mean? That makes zero sense.
There are tools in the software that will help you troubleshoot. Either use test under tools, or drop a single on effect at the node level. This will show you how xlights and real world lights line up. So, if you are commanding node 25 in the 4th strand and you don't see that on the tree, then you have mapping issues.