Combo is endless my tree is 24 strands 150 nodes 3600 total. I'm using 6 outputs so each output runs up and down the tree 4 times. I power inject with nothing off the controller but ground and data. so all outputs are on one side. I'm using 2 600 watt power supplies. with yours you are hitting about 240 watts. so 1 power supply and all off 1 port. You would be under the 80% mark on your power supply. You would still have to inject with out going through the controller because of the 20 amp mark. With your set up I would inject every 100 nodes. or you can fill all ports with no injection still only one power supply hooked to both sides of the controller. But that's 16 wires needed to be run. 18 gauge would work. my way need 14 gauge to the start node then daisy chain the ground and positive to every 100th node.
Just remember when you ask a question here you will get 6 different answers, They will all be right you just need to chose what is best for you.