If you see something lighting up in the preview and you've mapped channels correctly then the real lights will always match. You haven't really said how you are playing the sequence. Is it live playback in the sequencer tab, playback via the scheduler, playback via FPP? Have you done a Render All before you play it back if it's in the sequencer? Do you have Render On Save checked in options if you are playing back via a different method?